纳米孔测序技术在环境微生物研究中的应用

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高通量测序技术是研究环境微生物的有效手段,而以纳米孔测序为代表的第三代测序技术以其测序读长长、测序速度快、测序数据实时监控、仪器方便携带、无GC偏好性、无需经过PCR扩增等显著优势有力推动了环境微生物研究的发展.本文对纳米孔测序技术的技术原理和特点进行了简要概述,重点介绍了纳米孔测序技术在环境微生物扩增子测序、宏基因组测序、全基因组测序等领域的研究应用,并分析了纳米孔测序技术在环境微生物应用中的优势及存在的问题.
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