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[目的]研究心肌缺血不同时间段血浆和尿液中心脏型脂肪酸结合蛋白(hear-type fatty acid binding pro-tein,H-FABP)含量的变化规律,探讨其在早期诊断急性心肌梗死方面的价值。[方法]应用结扎兔心脏左冠状动脉制作急性心肌缺血动物模型,采用ELISA法检测心肌缺血不同时间段血浆和尿液中H-FABP的浓度。[结果]在兔心肌缺血15min时,血浆和尿液中H-FABP含量即有升高,并且随着心肌缺血时间延长,血浆和尿液中H-FABP含量皆逐渐增高,血浆中H-FABP浓度在缺血3h达最高,而尿液中H-FABP的浓度在缺血4h达最高值,与对照组相比均有显著性差异。[结论]血浆和尿液中H-FABP含量检测对早期心肌缺血诊断有意义。
[Objective] To investigate the changes of the content of heart-type fatty acid binding pro-tein (H-FABP) in plasma and urine at different time points of myocardial ischemia, and to explore its role in the early diagnosis of acute myocardial infarction value. [Method] The animal model of acute myocardial ischemia was created by ligating the left coronary artery in rabbit heart. The concentrations of H-FABP in plasma and urine at different time points of myocardial ischemia were detected by ELISA. [Result] The content of H-FABP in plasma and urine increased at 15min after myocardial ischemia in rabbits, and the content of H-FABP in plasma and urine increased gradually with the prolongation of myocardial ischemia. The content of H FABP concentration reached the highest at 3h after ischemia, while the concentration of H-FABP in urine reached the highest value at 4h after ischemia, which was significantly different from the control group. [Conclusion] The detection of H-FABP in plasma and urine is of significance for the early diagnosis of myocardial ischemia.