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WO噬菌体是侵染节肢动物体内感染的Wolbachia的细菌病毒,人们推测WO噬菌体可能参与了寄主遗传变异的过程。我们对采自中国境内4个地理种群(上海闵行、云南普洱、山东济宁和宁夏青铜峡)的灰飞虱Laodelphaxstriatellus1~2龄若虫用抗生素HCl-tetracycline处理过的水稻饲养,每隔20d取样测定并比较其体内Wolbachia和WO噬菌体的感染率,以此来初步研究灰飞虱体内WO噬菌体与Wolbachia的侵染关系,结果表明:WO噬菌体感染率的变化趋势与其宿主Wolbachia的基本一致,都随着时间推移逐步下降。我们进一步对未经HCl-tetracycline处理的灰飞虱,用实时定量PCR的方法对WO噬菌体和Wolbachia在不同日龄灰飞虱雌虫体内的菌量进行测定,结果显示,二者菌量都随着日龄的增长有所变化,在第8天达到最大,二者的变化趋势基本一致。由此我们推断WO噬菌体是侵染胞内共生菌Wolbachia的专性病毒,并且感染Wolbachia的WO噬菌体很可能是溶原性的噬菌体。
WO bacteriophage is a bacterial virus that infects Wolbachia that infects arthropods in vivo and it is speculated that WO bacteriophages may be involved in the genetic variation of the host. We fed on rice from 1 to 2 instar nymphs of Laodelphax striatellus, which were collected from 4 geographic populations in China (Minhang, Shanghai, Pu’er, Jining, Shandong and Qingtongxia, Ningxia) using antibiotic HCl-tetracycline and sampled every 20 days. The Wolbachia and WO phage infection rates were compared in order to study the relationship between Woolbachia infection and Woolbachia infection. The results showed that the trend of the infection rate of WO phage was basically the same as that of its host Wolbachia, Passing down gradually. We further investigated the phage of Woolbachia and WOB on the larvae of Laodelphax striatellus at different days by using real-time quantitative PCR. The results showed that both of them were The change of day length has been changed, reaching the maximum on the 8th day. The trend of the two changes is basically the same. From this we infer that the WO phage is an obligate virus that infects the intracellular symbionts Wolbachia and that the WO phages that are infected with Wolbachia are likely to be lysogenic phages.