Traditional Chinese herbs (TCH) are currently gaining attention in disease prevention and health care plans.However,their general bitter taste hinders their use.Despite the development of a variety of taste evaluation methods,it is still a major challenge to establish a quantitative detection technique that is objective,authentic and sensitive.Based on the two-bottle preference test (TBP),we proposed a novel quantitative strategy using a standardized animal test and a unified quantitative benchmark.To reduce the difference of results,the methodology of TBP was optimized.The relationship between the concentration of quinine and animal preference index (PI) was obtained.Then the PI of TCH was measured through TBP,and bitteess results were converted into a unified numerical system using the relationship of concentration and PI.To verify the authenticity and sensitivity of quantified results,human sensory testing and electronic tongue testing were applied.The quantified results showed a good discrimination ability.For example,the bitteess of Coptidis Rhizoma was equal to 0.0579 mg/mL quinine,and Nelumbinis Folium was equal to 0.0001 mg/mL.The validation results proved that the new assessment method for TCH was objective and reliable.In conclusion,this study provides an option for the quantification of bitteess and the evaluation of taste masking effects.