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目的:独一味注射液的抗炎作用的研究及其作用机制的初步探讨。方法:通过大鼠棉球肉芽肿模型和二甲苯所致小鼠耳肿胀模型,观察独一味注射液的抗炎作用;采用中性红吞噬试验研究独一味注射液对小鼠巨噬细胞的吞噬功能的影响,并采用小鼠胸腺细胞增殖法检测独一味注射液对小鼠巨噬细胞上清液中的白介素-1含量的影响。结果:独一味注射液在0.225和0.45g·kg-1剂量下能显著的抑制大鼠棉球肉芽肿的形成,在0.45,0.9和1.8g·kg-1剂量下能显著的抑制二甲苯所致的小鼠耳肿胀;独一味注射液显著的增强了巨噬细胞的吞噬能力,并且抑制了脂多糖诱导的白介素-1的分泌。结论:独一味注射液具有明显的抗炎作用,其作用机制可能与增强巨噬细胞的吞噬功能和抑制白介素-1的分泌有关。
Objective: To study the anti-inflammatory effect of Duoyitai Injection and to explore its mechanism. Methods: The anti-inflammatory effect of Duobuwei injection was observed by cotton granuloma rat model and mouse ear swelling model induced by xylene. Neutrophil phagocytosis was used to investigate phagocytosis of mouse macrophages Function, and the mouse thymocyte proliferation assay was used to detect the effect of DUOYI on IL-1 in mouse macrophage supernatant. Results: Only one injection at 0.225 and 0.45g · kg-1 dose significantly inhibited the formation of cotton ball granuloma in rats at 0.45,0.9 and 1.8g · kg-1 dose can significantly inhibit the xylene Induced mice’s ear swelling; Duyiwei injection significantly enhanced the phagocytic capacity of macrophages, and inhibited lipopolysaccharide-induced interleukin-1 secretion. Conclusion: Duoyitai injection has obvious anti-inflammatory effect, and its mechanism may be related to enhancing the phagocytosis of macrophages and inhibiting the secretion of interleukin-1.