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Pen a 1 is a major shrimp allergen that induces an allergy.The aim of this study is to assess the effect of simulated gastrointestinal fluids on the allergenicity of Pen a 1.Purified Pen a 1 from shrimp (Penaeus vannamei) was subjected to digestion in SGF (simulated gastric fluid) and SIF (simulated intestinal fluid) for a set time.The allergenicity of digestive Pen a 1 was analyzed by immunoblotting and Ci-ELISA,using pool sera from patients with shrimp specific IgE.The results showed that Pen a 1 exhibited a decrease in allergenicity with increasing digestion time in the SGF and SIF.However,Pen a l exhibited strong resistance to digestive fluids,and all yielded fragments (33 kD,23 kD,and 14 kD) showed allergic activity.Therefore,anti-digestion may be an important factor for Pen a 1 to induce an allergy.
Pen a 1 is a major shrimp allergen that induces an allergy. The aim of this study is to assess the effect of simulated gastrointestinal fluids on the allergenicity of Pen a 1. Purified Pen a 1 from shrimp (Penaeus vannamei) was subjected to digestion in SGF (simulated intestinal fluid) and SIF (simulated intestinal fluid) for a set time. The allergenicity of digestive Pen a 1 was analyzed by immunoblotting and Ci-ELISA, using pool sera from patients with shrimp specific IgE. The results showed that Pen a 1 showed a decrease in allergenicity with increasing digestion time in the SGF and SIF. However, Pen al means strong resistance to digestive fluids, and all yielded fragments (33 kD, 23 kD, and 14 kD) showed allergic activity. Wherefore, anti- digestion may be an important factor for Pen a 1 to induce an allergy.