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目的探讨国人乳腺癌中ＥＢ病毒ＤＮＡ（ＥＢＶ－ＤＮＡ）的检出率及ＥＢ病毒（ＥＢＶ）基因编码蛋白的表达情况。方法（１）在１０２例乳腺癌中用聚合酶链反应（ＰＣＲ）技术扩增ＥＢＶ内部重复序列ＢａｍＨＩＷ区域，并以３４例乳腺纤维腺瘤，３例管内乳头状瘤和１０例乳腺导管上皮增生作为对照组；（２）用ＡＢＣ免疫组织化学技术检测其中７３例乳腺癌中的ＥＢＶ潜在膜蛋白（ＬＭＰ）和ＥＢＶ核抗原２（ＥＢＮＡ２）。结果（１）ＰＣＲ检测显示２９例（２８．４％）乳腺癌有ＥＢＶ－ＤＮＡ，而对照组中仅２例（４．３％）纤维腺瘤有ＥＢＶ－ＤＮＡ，两组的差异在统计学上有显著意义（Ｐ＜０．００１）；（２）免疫组化技术显示ＬＭＰ和ＥＢＮＡ２阳性率分别为１３．７％和１７．８％，且阳性物质定位于肿瘤细胞，而非肿瘤中浸润的淋巴细胞。结论ＥＢＶ感染与乳腺癌的发病可能有一定关系，明确ＥＢＶ感染在乳腺癌发病机制中的作用将有十分积极的意义。
Objective To investigate the detection rate of Epstein-Barr virus DNA (EBV-DNA) in Chinese breast cancer and the expression of Epstein-Barr virus (EBV) gene-encoded protein. Methods (1) PCR amplification of EBV internal repeat BamHIW region in 102 cases of breast cancer, 34 cases of breast fibroadenoma, 3 cases of intraductal papilloma and 10 cases of breast ductal hyperplasia As a control group; (2) EBV latent membrane protein (LMP) and EBV nuclear antigen 2 (EBNA2) in 73 cases of breast cancer were detected by ABC immunohistochemical technique. Results (1) PCR showed 29 cases (28.4%) of breast cancer had EBV-DNA, while only 2 cases (4.3%) of fibroadenoma had EBV-DNA in control group. The difference between the two groups was statistically significant. Significantly (P<0.001); (2) Immunohistochemistry showed that the positive rates of LMP and EBNA2 were 13.7% and 17.8%, respectively, and the positive substance was localized in tumor cells but not in tumors. Lymphocytes. Conclusion EBV infection may have a certain relationship with the incidence of breast cancer. It is clear that the role of EBV infection in the pathogenesis of breast cancer will be of great significance.