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目的探讨恒温恒压电热烫伤仪制作糖尿病大鼠不同深度烫伤模型的可行性。方法链脲佐菌素诱导正常SD大鼠制作糖尿病大鼠模型,诱导成功1、2、3、4周检测皮肤晚期糖基化终产物、胶原含量,以正常SD大鼠作为对照,判断典型糖尿病性皮肤改变所需的时间。以恒温恒压烫伤仪制作烫伤模型,制模条件为0.5 kg压力下,80℃,分别作用时间4、6、12 s,48 h后取材行苏木素-伊红(HE)染色检测烫伤深度。结果糖尿病大鼠诱导成功后4周,皮肤晚期糖基化终产物含量为(31.40±3.45)U/mg、胶原含量为(12.60±0.57)mg/g,与正常对照组比较差异有统计学意义(P<0.05),并出现典型的糖尿病性皮肤改变。HE染色烫伤时间4、6、12 s时深度依次为浅Ⅱ度、深Ⅱ度和Ⅲ度。结论恒温恒压电热烫伤仪制作糖尿病大鼠不同深度烫伤模型简单、方便,重复性高,为研究糖尿病创面愈合提供较为理想的模型。
Objective To investigate the feasibility of using constant temperature and constant pressure electrothermal scald apparatus to make different depth scald models in diabetic rats. Methods STZ rats were induced by streptozotocin to induce the development of diabetic rat model. The advanced glycation end products (AGEs) and collagen contents of skin were measured at 1, 2, 3 and 4 weeks after successful induction. Normal SD rats were used as controls to determine the typical diabetes The time it takes for the skin to change. The scald model was made by constant temperature and constant pressure scald instrument. The molding conditions were 0.5 kg pressure, 80 ℃, 4 h, 12 h and 48 h respectively. The hematoxylin and eosin (HE) staining was used to detect the scald depth. Results The level of advanced glycosylation end products was (31.40 ± 3.45) U / mg and the content of collagen was (12.60 ± 0.57) mg / g in diabetic rats 4 weeks after successful induction, which was significantly different from the normal control (P <0.05), and typical diabetic skin changes. The depth of hematoxylin and eosin staining at 4, 6 and 12 s were as follows: shallow Ⅱ, deep Ⅱ and Ⅲ. CONCLUSION: The constant temperature and constant pressure electrothermal scald instrument makes the model of diabetic rats with different depth scalds with simple, convenient and high repetitiveness. It provides an ideal model for studying the healing of diabetic wounds.