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目的探讨转化生长因子(TGF)-β1在系统性红斑狼疮(SLE)患者中的表达和临床意义。方法采用双抗体夹心酶联免疫吸附试验(ELISA)检测12例SLE血小板减少患者、9例血小板正常患者和12名健康对照者血清中的TGF-β1水平。SLE病例,用光学显微镜分类计数标准化骨髓涂片中的巨核细胞。结果SLE血小板减少组血清中TGF-β1浓度(3.63～32.84μg/L,中位数为10.64μg/L)和SLE血小板正常组患者血清中TGF-β1浓度(2.43～26.51μg/L,中位数为18.03μg/L)均减少,低于正常对照组(11.95～39.51μg/L,中位数为27.53μg/L),差异有统计学意义(P<0.05);SLE伴血小板减少组骨髓涂片均为巨核细胞计数正常或增多,颗粒巨核细胞增多,产血小板巨核细胞减少,伴有明显的巨核细胞成熟障碍。结论TGF-β1没有直接参与SLE血小板减少患者巨核细胞的负性调节,但TGF-β1作为一种强烈的免疫抑制因子,可能参与了SLE的发病机制。
Objective To investigate the expression and clinical significance of transforming growth factor-β1 (TGF-β1) in patients with systemic lupus erythematosus (SLE). Methods Serum levels of TGF-β1 in 12 patients with SLE, 9 patients with normal platelet and 12 healthy controls were detected by ELISA. SLE cases, the megakaryocytes in the standard marrow smear were sorted by light microscopy. Results Serum concentrations of TGF-β1 (3.63-32.84μg / L, median 10.64μg / L) and serum TGF-β1 (2.43-26.51μg / L) in SLE group were significantly higher than those in SLE group (P <0.05). The number of bone marrow of patients with SLE with thrombocytopenia was significantly lower than that of normal controls (11.95-39.51μg / L, median 27.53μg / L) Smears were all normal or increased megakaryocyte counts, increased number of megakaryocytes, platelet megakaryocytes decreased, with obvious megakaryocyte maturation disorder. Conclusion TGF-β1 is not directly involved in the negative regulation of megakaryocytes in patients with SLE, but TGF-β1, as a strong immunosuppressive factor, may be involved in the pathogenesis of SLE.