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目的 :研究肿瘤坏死因子α(TNFα)和血管紧张素Ⅱ (AngⅡ )对心肌细胞的细胞间粘附分子 - 1(ICAM - 1)表达的不同影响。方法 :运用免疫组化技术和流式细胞仪观察TNFα和AngⅡ对培养的乳鼠心肌细胞ICAM - 1表达量的影响。结果 :TNFα明显增加心肌细胞ICAM - 1表达量 ,以刺激 6h最明显 ,随时间作用延长表达量减少。AngⅡ对培养的心肌细胞ICAM - 1表达量无显著作用。结论 :TNFα是调节心肌细胞ICAM - 1表达的重要细胞因子。AngⅡ并没有调节心肌细胞ICAM - 1表达的作用
Objective: To study the different effects of tumor necrosis factor α (TNFα) and angiotensin Ⅱ (Ang Ⅱ) on the expression of ICAM - 1 in cardiomyocytes. Methods: The effects of TNFα and Ang Ⅱ on the ICAM - 1 expression in neonatal rat cardiomyocytes were observed by immunohistochemistry and flow cytometry. Results: TNFα significantly increased the expression of ICAM - 1 in cardiomyocytes, and the expression of ICAM - 1 was the most obvious at 6h after stimulation. Ang Ⅱ had no significant effect on the expression of ICAM - 1 in cultured cardiomyocytes. Conclusion: TNFα is an important cytokine that regulates ICAM - 1 expression in cardiomyocytes. Ang Ⅱ did not regulate the expression of ICAM - 1 in cardiomyocytes