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目的 :采用神经电生理检查方法 ,观察逆转录病毒载体介导脑源性神经营养因子 ( BDNF)基因修饰成肌细胞对损伤脊髓的治疗作用。 方法 :3 0只 SD大鼠在 T9水平制成脊髓横断损伤模型 ,并随机分为基因细胞组 ( A组 )、成肌细胞组 ( B组 )及损伤对照组 ( C组 ) ,每组 10只大鼠。术后 3个月 ,采用皮质体感诱发电位 ( CSEP)和运动诱发电位 ( MEP)等电生理检测技术 ,观察轴突是否有再生及其神经功能恢复程度。 结果 :( 1) A组中 3只大鼠损伤 3个月后出现 CSEP波 ,5只出现 MEP波 ,B,C组动物未发现电生理信号恢复 ;( 2 )重新出现的 CSEP或 MEP信号均较损伤前波幅减低 ,潜伏期延长。 结论 :BDNF基因修饰细胞脊髓内移植能有效促进损伤的脊髓神经部分传导功能恢复
OBJECTIVE: To observe the therapeutic effect of retroviral vector-mediated BDNF gene myoblasts on injured spinal cord by electrophysiological method. Methods: 30 Sprague-Dawley rats were made spinal cord transection injury model at T9 level and randomly divided into group A (gene cell), group B (myoblast) and group C (group C) Only rats. At 3 months after operation, electrophysiological detection techniques such as cortical somatosensory evoked potential (CSEP) and motor evoked potential (MEP) were used to observe whether axon regeneration and neurological function recovery were observed. Results: (1) CSEP wave appeared in 3 rats in group A and MEP wave appeared in 3 rats in group A, and no electrophysiological signal recovery was found in animals in groups B and C; (2) The reappearance of CSEP or MEP signal Lower amplitude than before injury, the incubation period is prolonged. CONCLUSION: Transplantation of BDNF gene-modified cells into the spinal cord can effectively promote the restoration of spinal cord nerve conduction function