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目的:研究荔枝核的提取工艺,建立HPLC法测定荔枝核中原儿茶酸含量。方法:L9(34)正交设计,探讨加水倍数、煎煮时间和煎煮次数对荔枝核提取的影响;采用色谱柱Symmetry C18;流动相为甲醇-水-冰乙酸(10∶118∶1);检测波长为260nm。结果:荔枝核提取时以10倍量水煎煮3次,每次0.5 h;醇沉纯化浓度为60%;原儿茶酸在5.04~50.4μg.mL-1呈良好线性关系(r=0.999 7),平均加样回收率为97.70%,RSD=1.69%。结论:加水倍数和煎煮次数对荔枝核中原儿茶酸的提取有显著性影响,HPLC测定方法简单快速,适用性好。
Objective: To study the extraction technology of litchi nucleus and establish HPLC method for the determination of protocatechuic acid in litchi nucleus. Methods: L9 (34) orthogonal design was used to investigate the effect of multiple times of boiling, boiling time and decoction times on the extraction of litchi. The chromatographic column Symmetry C18 was used. The mobile phase consisted of methanol-water and glacial acetic acid (10:11 8:1) ; Detection wavelength of 260nm. Results: The litchi nucleus was decocted with 10 times of water for 3 times for 0.5 h each time, and the concentration of alcohol precipitation was 60%. The calibration curve of protocatechuic acid was linear in 5.04 ~ 50.4 μg.mL-1 (r = 0.999 7), the average recovery was 97.70%, RSD = 1.69%. Conclusion: The times of adding water and decocting times have significant effects on the extraction of protocatechuic acid in litchi nucleus. The method of HPLC determination is simple and rapid and the applicability is good.