论文部分内容阅读
目的检测EphB4蛋白和微血管密度（MVD）在结直肠癌组织中的表达情况及它们与结直肠癌生物学特性的关系。方法选取手术切除并经病理证实的结直肠癌标本63例、正常结直肠组织25例，应用免疫组织化学法检测EphB4蛋白的表达及MVD水平。结果（1）EphB4蛋白在结直肠癌组织中的阳性率为65.1%（41/63），正常组织中则为28.0%（7/25），组间比较差异有统计学意义（P＜0.05）；EphB4蛋白阳性表达的结直肠癌组织中MVD为41.00±7.76,阴性表达组为32.91±10.27，EphB4蛋白阳性表达组MVD水平高于阴性表达组（P＜0.05）。（2）结直肠癌组织中EphB4蛋白的表达与肿瘤分化程度、浸润深度、淋巴转移及Dukes分期有密切关系（P＜0.05），而与年龄、性别、肿瘤大小及部位无明显相关性（P＞0.05）。结论 EphB4蛋白是反映结直肠癌进展程度的重要指标，与肿瘤的生长及转移密切相关。“,” Objective To investigate the expression of EphB4 and microvessel density(MVD)levels in colorectal carcinoma and to analyze the relationship between the expression of EphB4 and the bionomics of colorectal carcinoma. Methods Sixty-three specimens from colorectal carcinoma diagnosed by pathology were examined by immunohistochemistry for EphB4 and MVD.25 specimens from normal colorectal tissue were examined as controls.Results The expression of EphB4 protein was 65.1%(41/63) for colorectal carcinoma, 28.0% (7/25) for the normal colorectal tissues, the difference was significant ( P<0.05) ; Meanwhile MVD was significantly higher in EphB4 positive group (41.0±7.76)than that in EphB4 negative group(32.91±10.27)( P<0.05).(2)The expression of EphB4 protein had a significant positive correlation with differentiation grade,infiltration,lymph node metastases and clinical stage( Objective To investigate the expression of EphB4 and microvessel density(MVD)levels in colorectal carcinoma and to analyze the relationship between the expression of EphB4 and the bionomics of colorectal carcinoma. Methods Sixty-three specimens from colorectal carcinoma diagnosed by pathology were examined by immunohistochemistry for EphB4 and MVD.25 specimens from normal colorectal tissue were examined as controls.Results The expression of EphB4 protein was 65.1%(41/63) for colorectal carcinoma, 28.0% (7/25) for the normal colorectal tissues, the difference was significant ( P<0.05) ; Meanwhile MVD was significantly higher in EphB4 positive group (41.0±7.76)than that in EphB4 negative group(32.91±10.27)( P<0.05).(2)The expression of EphB4 protein had a significant positive correlation with differentiation grade,infiltration,lymph node metastases and clinical stage(P0.05). Conclutions The EphB4 protein expression increase may be involved in development of colorectal carcinoma and play important roles in the tumorigenesis and progression of colorectal carcinoma.