论文部分内容阅读
[目的]研究复肝宁治疗肝纤维化(HF)的效果及部分作用机制。[方法]采用猪血清诱导免疫性HF动物模型。70只大鼠随机分为6组:正常组,模型组,秋水仙碱组,复肝宁低、中、高剂量组。造模6周后,各组大鼠给予相应药物或0.85%氯化钠灌胃。实验10周后结束,检测血清丙氨酸氨基转移酶(ALT),天冬氨酸转氨酶(AST),透明质酸(HA),层黏连蛋白(LN),Ⅳ型胶原(Ⅳ-C)。[结果]与正常组比较,模型组大鼠血清ALT、AST、HA、LN、Ⅳ-C水平明显升高(P<0.01)。复肝宁高、中、低剂量组及秋水仙碱组均可降低血清ALT、AST水平,且与模型组比较均有统计学意义(P<0.01)。复肝宁高、中剂量组及秋水仙碱组HA、Ⅳ-C、LN与模型组比较差异均有统计学意义(P<0.01);复肝宁低剂量组LN与模型组比较P<0.05,HA、Ⅳ-C与模型组比较P>0.05。[结论]复肝宁对猪血清诱导的HF大鼠有确切治疗作用,其机制可能是通过保护肝细胞,抑制胶原合成来实现的。
[Objective] To study the effect and partial mechanism of Fuganning on liver fibrosis (HF). [Method] Porcine serum was used to induce immune HF animal model. 70 rats were randomly divided into 6 groups: normal group, model group, colchicine group, Fuganning low, medium and high dose groups. After 6 weeks of modeling, the rats in each group were given the corresponding drugs or gavage with 0.85% sodium chloride. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), hyaluronic acid (HA), laminin (LN) and collagen Ⅳ (Ⅳ- . [Results] Compared with the normal group, the levels of serum ALT, AST, HA, LN and Ⅳ-C in the model group were significantly increased (P <0.01). The levels of ALT and AST in serum of patients with high, medium and low doses of Fuganning, and those of colchicine group were lower than those of the model group (P <0.01). Compared with the model group, the differences of HA, Ⅳ-C and LN in the model group were statistically significant (P <0.01); the difference between the model group and the low-dose Fuganning group was significant (P <0.05) , HA, Ⅳ-C compared with the model group P> 0.05. [Conclusion] Fuganning has an exact therapeutic effect on porcine serum-induced HF in rats. The mechanism may be achieved by protecting hepatocytes and inhibiting collagen synthesis.