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目的;探讨琥珀酰缩胆囊素七肽(SCH)对小鼠海马突触体摄取~(45)Ca的影响,并与辛卡利特作.了比较.方法:蛋白质浓度的测定用染料法.~(45)Ca的摄取测定采用~(45)CaCl_2加入突触体悬液中孵育后用玻璃微纤维过滤器过滤,用液闪光谱仪计数.结果:SCH是辛卡利特异构物.实验表明在0.125—2 μmol·L~(-1)的剂量范围内SCH呈剂量依赖性地抑制小鼠海马突触体摄取~(45)Ca.其抑制的程度与辛卡利特近似.SCH与辛卡利特的这种抑制效应能被丙谷胺、β-内啡肽所拮抗.结论:辛卡利特N端天冬氨酸残基的改变对海马突触体摄取~(45)Ca无明显影响.
Objective To investigate the effect of succinyl cholecystokinin (SCH) on uptake of 45 Ca in hippocampal synapse of mice and compare with that of Xinacalit.Methods: The protein concentration was determined by the dye method. The uptake of Ca (45) Ca was determined by the liquid flash spectrometer after adding 45 CaCl2 into the synaptosome suspension and filtering with glass microfiber filter.Results: In a dose range of 0.125-2 μmol·L -1, SCH inhibited the uptake of 45 Ca into the hippocampal synaptosomes in a dose-dependent manner in SCH. The inhibitory effect of simmalitabine was antagonized by proglumide and β-endorphin.Conclusion: The change of N-terminal aspartic acid in ciclopirox could inhibit the uptake of ~ (45) Ca No significant effect.