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在pH=9.26的氨-氯化铵缓冲溶液中,铽Ⅲ与环丙沙星反应形成络合物,而后加入十二烷基硫酸钠(SDS)形成了三元体系,其最佳激发、发射波长分别为λex=330nm、λem=545 nm。在该反应体系中加入适量雷公藤红素溶液,铽Ⅲ与环丙沙星络合物的激发、发射波长位置不变,但荧光强度有规律下降。利用这一现象,建立了测定雷公藤红素的荧光分析方法。雷公藤红素浓度在7.2×10-6～4.4×10-5mol/L范围内符合线性关系;方法检出限为3.1×10-8mol/L。
Terbium Ⅲ reacted with ciprofloxacin to form a complex in ammonia-ammonium chloride buffer at pH = 9.26. Then, sodium dodecyl sulfate (SDS) was added to form a ternary system. The best excitation and emission The wavelengths are λex = 330 nm and λem = 545 nm, respectively. In the reaction system, adding appropriate amount of tripterine solution, terbium Ⅲ and ciprofloxacin excitation, the emission wavelength position unchanged, but the fluorescence intensity decreased regularly. Using this phenomenon, the fluorescence assay for the determination of tripterine was established. Tripterine concentration in the 7.2 × 10-6 ~ 4.4 × 10-5mol / L range in line with the linear relationship; method detection limit of 3.1 × 10-8mol / L.