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背景:光学相干层析成像技术是一种高分辨率、非创伤性成像技术,它能够利用近红外光产生牙体组织的深度解析图像,并通过检测背向散射信号来对龋损进行定量检测。目的:通过与偏振光显微镜的对比,验证光学相干层析成像技术对光滑面早期釉质龋的成像能力及对龋损深度定量检测的准确性。方法:以6岁龄牛下切牙为样本,采用pH循环法建立光滑面早期龋人工模型,分别应用全光纤光学相干层析成像技术和偏振光显微镜进行检测,比较两种方法获得龋损图像和深度值,进行方差分析和相关性分析。结果与结论:光学相干层析成像系统能够清晰成像光滑面早期龋,表层下脱矿区域信号显著增强,并且测得的脱矿深度值与偏振光显微镜结果一致,即该系统对深度的测量具有较高的准确性,可用于对早期龋纵深进展的评估。光学相干层析成像技术测得的人工龋模型开窗区的深度值与脱矿时间无相关性(P>0.05)。计算开窗区平均深度值与开窗区外平均深度值的差值即深度变化值(△D),则△D与脱矿时间呈正相关(r=0.954,P<0.05)。结果提示了这种无创、高分辨率的光学成像技术在早期龋的定量研究和临床诊断方面具有广阔的应用前景。
BACKGROUND: Optical coherence tomography is a high-resolution, non-invasive imaging technique that uses near-infrared light to generate depth-resolved images of dental tissues and quantify carious lesions by detecting backscatter signals . OBJECTIVE: To verify the ability of optical coherence tomography to detect the early enamel caries in smooth surface and the accuracy of quantitative detection of carious lesions by comparing with polarized light microscopy. Methods: A 6-year-old incisor model was established. Artificial smooth muscle early caries model was established by pH cycle method. All-fiber optical coherence tomography and polarized light microscope were used to detect the caries. Depth values, variance analysis and correlation analysis. RESULTS AND CONCLUSION: The optical coherence tomography system can clearly image the early caries in the smooth surface and the signals in the sub-surface demineralization area are significantly enhanced. The measured demineralization depth is consistent with the result of the polarization microscope. That is, the system has the following features The higher accuracy can be used to assess the depth of early caries progression. There was no correlation between the depth of fenestration and the demineralization time of the artificial caries model measured by optical coherence tomography (P> 0.05). Calculate the difference between the average depth of the fenestration area and the average depth of the window outside the window that is the depth of change (△ D), then △ D and demineralization time was positively correlated (r = 0.954, P <0.05). The results suggest that this non-invasive, high-resolution optical imaging technique has broad application prospects in the quantitative study and clinical diagnosis of early caries.