以正己烷与丁酮组成的混合溶剂体系,以Novozym435固定化酶为催化剂,以果糖和棕榈酸为反应底物合成糖酯。研究不同溶剂比例对反应的影响,及在最佳溶剂体系中对反应条件的优化摸索,实验结果表明:当丁酮在混合溶剂中的体积含量为80%(logP=0.74),pH为7.5、反应温度为50℃、果糖与棕榈酸的摩尔比为1∶1.5、酶浓度为15g/L时,产物转化率可达到85%。 A mixed solvent system consisting of n-hexane and methyl ethyl ketone was used to synthesize sugar esters using Novozym 435 immobilized enzyme as catalyst and fructose and palmitic acid as reaction substrates. The effects of different solvent ratios on the reaction were studied, and the optimum reaction conditions were optimized. The experimental results showed that when the volume fraction of butanone in the mixed solvent was 80% (logP = 0.74), the pH was 7.5, The reaction temperature was 50 ℃, the molar ratio of fructose and palmitic acid was 1: 1.5, and the enzyme conversion was 15%.