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目的 :探讨高蛋白高胆固醇饮食诱导心肌纤维化的协同效应及其发生机制。方法 :在每日标准饮食中增加2 0 %蛋白质或 /和 10 0mg胆固醇摄入 8周的大鼠 ,以羟脯氨酸法测心肌胶原含量 ;以放免法测左心室及血浆AngⅡ和Ald浓度 ;以Griess法测血清亚硝酸盐 (NO-2 )浓度。结果 :高蛋白高胆固醇组较高蛋白组心肌胶原含量升高了1.6 9倍 ,血总胆固醇和AngⅡ浓度分别升高了 0 .7倍和 1.5倍 ,血NO-2 浓度亦明显降低 ,心肌Ald含量上升了 1倍 ;较高胆固醇组心肌胶原含量升高了 0 .4 8倍 ,血AngⅡ升高了 0 .2 3倍。 结论 :高蛋白高胆固醇饮食可协同诱导心肌纤维化 ,其发生机制可能与RAAS激活和内皮功能受损有关。
Objective: To investigate the synergistic effect and its mechanism of myocardial fibrosis induced by high protein and cholesterol diet. Methods: The rats were fed with 20% protein or / and 10 mg cholesterol for 8 weeks. The content of myocardial collagen was measured by hydroxyproline. The concentrations of Ang Ⅱ and Ald in the left ventricle and plasma were measured by radioimmunoassay Serum nitrite (NO-2) concentration was measured by Griess method. Results: Compared with the high protein high cholesterol group, the content of myocardial collagen increased by 1.6 9 times, the total cholesterol and Ang Ⅱ concentration increased 0.7 times and 1.5 times respectively, and the blood NO-2 concentration was also significantly decreased. The content increased by 1 times; the higher cholesterol group increased 0.48 times the level of myocardial collagen, blood Ang Ⅱ increased 0.23 times. Conclusion: High protein and high cholesterol diet can synergistically induce myocardial fibrosis. The mechanism may be related to RAAS activation and impaired endothelial function.