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对恶性疟原虫的基因图谱和由遗传操作产生的突变疟原虫的分析需要一种快速、灵敏的方法来鉴定疟原虫克隆。克隆时一般用未感染的红细胞稀释疟原虫,使96孔培养板每孔中感染红细胞数不到一个。培养3周后,涂片用吉氏液染色,镜检疟原虫。用此法虽然可以使原虫浓度低于0.1％,但很费时,仅一块培养板就需花费至少一天的时间分析。Kirkman等根据疟原虫浓度大于0.5％时,
Analysis of the P. falciparum genetic map and the mutated Plasmodium that is produced by genetic manipulation requires a rapid and sensitive method to identify the Plasmodium clones. Clones are generally diluted with uninfected erythrocytes to reduce the number of infected red blood cells per well in a 96-well plate. After 3 weeks of culture, the smears were stained with Giemsa and the plasmids were examined microscopically. Although this method can make protozoa concentration less than 0.1%, but it is time-consuming, it takes at least a day to analyze only one plate. Kirkman, etc. According to the concentration of Plasmodium is greater than 0.5%