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目的探讨纳米磁小体药囊靶向治疗肝癌的机制。方法肝癌裸鼠模型32只,记录各组治疗前及连续5d治疗后1、4、7、10、13d肿瘤体积,免疫组织化学和逆转录-聚合酶链反应(RT-PCR)检测bcl-2、bax、半胱氨酸蛋白酶-3(Caspase-3)蛋白和基因表达。结果纳米磁小体药囊结合内置支架组肿瘤生长受到明显抑制,治疗13d后各组肿瘤体积分别为(367.8±44.0)、(465.0±91.2)、(764.7±100.6)、(776.1±116.2)mm~3,其体积与其他各组差异有统计学意义(P<0.05),bcl-2的蛋白和mRNA表达(0.154±0.014)也低于其他各组(0.275±0.025、0.524±0.049、0.557±0.064)(P<0.01);bax、Caspase-3的蛋白和mRNA表达则高于其他各组(P<0.01)。结论纳米磁小体药囊对肝癌裸鼠移植瘤有明显抑制作用,下调bcl-2表达和对bax、Caspase-3的表达上调可能是其抑瘤机制之一。
Objective To investigate the mechanism of nano magnetosome drug capsule targeting liver cancer. Methods Twenty-two hepatocellular carcinoma (HCC) nude mice models were established. The tumor volumes at 1, 4, 7, 10 and 13 days after treatment were recorded before and 5 days after treatment. Immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR) , Bax, Caspase-3 protein and gene expression. Results The growth of tumor was significantly inhibited by nano-magnetosome combined with built-in scaffold. The tumor volume of each group was (367.8 ± 44.0), (465.0 ± 91.2), (764.7 ± 100.6), (776.1 ± 116.2) mm (P <0.05). The protein and mRNA expression of bcl-2 (0.154 ± 0.014) were also lower than other groups (0.275 ± 0.025, 0.524 ± 0.049 and 0.557 ± 0.064) (P <0.01). The protein and mRNA expression of bax and Caspase-3 were higher than other groups (P <0.01). Conclusion Nanomaterials capsule can significantly inhibit the transplanted hepatocellular carcinoma in nude mice. Down-regulation of bcl-2 expression and up-regulation of bax and Caspase-3 may be one of the mechanisms of its inhibition.