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目的:通过对比黄连乌梅药对的HPLC指纹图谱,探讨该药对的配伍原理。方法:以色谱柱:AgilentHC-C18柱(4.6mm×250mm,5μm);检测波长:230nm;柱温:25℃为色谱条件,流动相:乙腈(A)-0.02mol/L磷酸二氢钾0.2%磷酸(B)梯度洗脱;检测波长:230nm;柱温:25℃为色谱条件,建立黄连乌梅药对的指纹图谱。结果:以黄连、乌梅单煎液作为对照,确定了13个共有峰,建立了不同配伍比例的指纹图谱。结论:乌梅与黄连的配伍比例趋近于1时,黄连中的盐酸药根碱、巴马汀、小檗碱溶出率达到最大。
OBJECTIVE: To compare the HPLC fingerprinting of Coptis chinensis and its compatibility principle. Method: Column: AgilentHC-C18 column (4.6mm × 250mm, 5μm); detection wavelength: 230nm; column temperature: 25 ℃ for chromatographic conditions; mobile phase: acetonitrile (A) -0.02mol / L potassium dihydrogen phosphate 0.2 % Phosphoric acid (B) gradient; detection wavelength: 230nm; column temperature: 25 ℃ for the chromatographic conditions, the establishment of Coptis Chinensis Pharmacopoeia fingerprinting. Results: With Coptis chinensis and Ebony decoction as control, 13 common peaks were identified, and fingerprints with different compatibility ratios were established. Conclusion: When the compatibility ratio of Fructus Mume and Coptis approximates to 1, the dissolution rates of jatrorrhizine hydrochloride, palmatine and berberine in Coptis chinensis reach the maximum.