论文部分内容阅读
目的:探讨各种化疗药物对高表达γ突触核蛋白(SNCG)的乳腺癌T47D细胞株的抑制作用。方法:常规培养不表达及高表达SNCG的乳腺癌MCF-7及T47D细胞株,采用MTT法观察顺铂(DDP)、阿霉素(ADM)、5-氟尿嘧啶(5-FU)、长春新碱(VCR)、紫杉醇(TAX)、依立替康(CPT-11)对细胞的抑制作用,并用流式细胞术分析药物作用后的细胞周期变化及细胞凋亡。结果:作用60 h后,ADM、VCR、TAX及CPT-11对T47D细胞的抑制作用显著低于MCF-7细胞(P<0.01),而DDP、5-FU对T47D细胞的抑制作用与MCF-7细胞相比差异无显著性(P>0.05),且显著高于其他4种抗肿瘤药物(P<0.01)。药物作用T47D细胞60 h后,DDP、5-FU组的细胞增殖指数(PI)及Caspase-3表达均较对照组及其他4组差异有极显著性(P<0.01)。结论:DDP及5-FU对高表达SNCG的T47D细胞的抑制作用显著高于ADM、VCR、TAX及CPT-11,此可为临床对乳腺癌患者个体化治疗方案的选择提供体外实验依据。
Objective: To investigate the inhibitory effect of various chemotherapeutic drugs on human breast cancer T47D cell line with high expression of γ-synuclein (SNCG). Methods: Breast cancer MCF-7 and T47D cell lines that did not express SNCG and high expression of SNCG were cultured routinely. The expressions of DDP, ADM, 5-FU and vincristine (VCR), paclitaxel (TAX) and irinotecan (CPT-11). The cell cycle and apoptosis were analyzed by flow cytometry. RESULTS: After 60 h treatment, the inhibitory effects of ADM, VCR, TAX and CPT-11 on T47D cells were significantly lower than those of MCF-7 cells (P <0.01), while the inhibitory effects of DDP and 5-FU on T47D cells were similar to those of MCF- 7 cells was no significant difference (P> 0.05), and was significantly higher than the other four antitumor drugs (P <0.01). The drug-induced T47D cells 60h, DDP, 5-FU group of cell proliferation index (PI) and Caspase-3 expression compared with the control group and the other 4 groups were significantly different (P <0.01). CONCLUSION: The inhibitory effect of DDP and 5-FU on T47D cells with high expression of SNCG is significantly higher than that of ADM, VCR, TAX and CPT-11. This study provides an experimental basis for the clinical selection of individualized treatment regimens for patients with breast cancer.