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目的探讨IGF-Ⅰ基因多态性与大于胎龄儿及脐血IGF-Ⅰ血清水平的关系,为降低大于胎龄儿的发生率提供理论依据。方法采用病例对照的研究方法,在南京地区收集大于胎龄儿(LGA)和适于胎龄儿(AGA)各96名,应用聚合酶链反应—限制性片段长度多态性方法(PCR-RFLP)检测IGF-Ⅰrs6214及rs35767的多态性,采用单因素Logistic回归分析计算LGA的相对危险度,比较2组间不同基因型出生时体格指标和脐血IGF-Ⅰ水平的差异。结果在LGA组和AGA组中,IGF-Ⅰrs35767 TT基因型可能会降低大于胎龄儿的风险(OR=0.235,95%CI=0.081~0.682),携带TT基因型个体的体重、头围和孕周均低于CC及CT基因型携带者;IGF-Ⅰrs6214 AG/GG基因型可能会增加大于胎龄儿的风险(OR=2.117,95%CI=1.151~3.894)。未发现2个多态性位点的改变与脐血IGF-Ⅰ血清水平相关。结论 IGF-Ⅰrs6214和rs35767多态性均与大于胎龄儿的发生相关。
Objective To investigate the relationship between the polymorphism of IGF-Ⅰ gene and serum IGF-Ⅰ levels in gestational age and umbilical cord blood, and to provide a theoretical basis for reducing the incidence of gestational age. Methods A case-control study was conducted in which 96 cases of LGA and AGA were collected from Nanjing and PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) ) Was used to detect the polymorphisms of IGF-Ⅰ rs6214 and rs35767. The relative risk of LGA was calculated by univariate Logistic regression analysis. The differences of physical indicators at birth and cord blood IGF-Ⅰ between different genotypes were compared. Results TT genotype of IGF-Ⅰ rs35767 in LGA group and AGA group may reduce the risk of being older than gestational age (OR = 0.235, 95% CI = 0.081-0.682). The TT genotype TT body weight, Weekly lower than CC and CT genotype carriers; IGF-Ⅰ rs6214 AG / GG genotype may increase the risk of larger than gestational age children (OR = 2.117,95% CI = 1.151 ~ 3.894). No changes in the two polymorphic sites were found to be associated with IGF-I serum levels in umbilical cord blood. Conclusion The polymorphisms of IGF-Ⅰ rs6214 and rs35767 are all associated with the occurrence of fetuses larger than gestational age.