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OBJECTIVE:To investigate the efficacy of the full composition granules of Huanglian(Rhizoma Copti-dis)(FGC)on the serum monocyte chemotactic pro-tein-1(MCP-1)and connective tissue growth fac-tor(CTGF)levels and kidney nuclear factor-KB(NF-κB)expression in rats with high-fat diet-in-duced diabetes.METHODS:Diabetes was induced in rats by feed-ing a high-fat chow combined with intravenous streptozotocin injection.Forty diabetic Sprague-Dawley rats were randomly assigned to a normal group(NG),model group(MG),irbesartan group(IG),and low-,middle-,and high-dosage FGC groups(LFGC,MFGC,HFGC),with eight rats per group.The IG rats received 31.25 mg·kg-1·d-1 irbe-sartan tablets,whereas those in the LFGC,MFGC,and HFGC were administered 52,312.5,and 625 mg·kg-1·d-1 FGC,respectively.After 12 weeks,bodyweight(BW),left kidney weight(KW),hemo-globin A1c(HbA1c),serum creatinine(Scre),blood urea nitrogen(BUN),and serum MCP-1 and CTGF levels were determined,pathological changes of the kidney were recorded,and kidney NF-κB p65(A)expression was measured.RESULTS:The 24-h urine albumin and levels of HbA1c,Scre,BUN,and serum MCP-1 and CTGF were significantly increased in in the MG compared with the NG,as was the kidney NF-κB(p65)expres-sion(P＜0.05).Furthermore,clear pathological changes in kidney fibrosis were observed in the MG rats.Following irbesartan and FGC administration,the 24-h urine albumin and the levels of HbA1c,Scre,and serum MCP-1 and CTGF were significantly decreased in FCG groups compared with those in the MG,which is in agreement with the change in the kidney NF-κB(p65)expression,whereas the similarly significant decrease only exist in 24-h urine albumin and the levels of serum CTGF after ir-besartan administration.Hematoxylin-eosin(HE)staining results indicated that the fibrosis observed in the MG samples was alleviated through FGC treatment.CONCLUSION:FGC may alleviate potential kidney injury by decreasing the serum MCP-1 and CTGF levels and inhibiting NF-kB expression in diabetic nephropathy in rats with high-fat diet-induced dia-betes.