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目的:研究铁皮石斛组培苗驯化的几个关键因素,为优质组培苗的大规模驯化提供技术依据。方法:以铁皮石斛组培苗为实验材料,探索季节气温、基质种类、基质厚度、定植方式和适应性锻炼对驯化苗成活的影响,及采用不同的方法诱捕基质中的蜗牛、蛞蝓,选择出最佳的驯化方案。结果:以松树皮碎片作基质时较好的驯化季节是夏季(盛夏除外)、秋季,较合适的基质厚度为6 cm左右;水苔作基质的驯化效果明显优于松树皮;穴盘驯化的效果明显优于苗床;适应性锻炼能克服初春和盛夏驯化组培苗的不利影响。白菜叶蘸取蔗糖水对蜗牛、蛞蝓的诱捕效果最好。结论:研究结果为规范铁皮石斛组培苗的驯化技术提供参考依据。
Objective: To study the domestication of Dendrobium candidum tissue culture of several key factors for the large-scale acclimation of tissue culture to provide technical basis. Methods: Tissue culture seedlings of Dendrobium candidum were used as experimental materials to explore the effects of seasonal temperature, substrate types, substrate thickness, planting methods and adaptive exercises on the survival of domesticated seedlings. Different methods were used to trap snails and slugs in the matrix, The best domesticated program. Results: The best domestication season in summer was pine (except in midsummer) when the pine bark fragments were used as the substrate. In autumn, the suitable substrate thickness was about 6 cm. The domestication of the moss as substrate was better than that of the pine bark. The effect is obviously better than the seedbed; adaptive exercise can overcome the adverse impact of early spring and summer acclimation of tissue culture seedlings. Cabbage leaves dipped in sugar water snail, catfish trap the best. Conclusion: The results provide reference for standardizing the domestication of tissue culture seedlings of Dendrobium candidum.