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本文用高亲和力、高特异性的人重组γ-干扰素(rhIFN-γ)单克隆抗体A_3,建立的人γ-干扰素的免疫放射量度分析。检测范围为0.10～250U/ml.批内和批间变异系数分别为7.40%和17.3%,回收率为1±0.17,稀释试验符合线性关系(r=0.98),不与IFN-α,IFN-β交叉。用本法检测了29例正常人、34例肿瘤病人和19例肿瘤化疗病人外周血单个核细胞诱生IFN-γ的量。结果表明肿瘤病人和正常人外周血单个核细胞产生IFN-γ的能力无明显差别,化疗病人外周血单个核细胞产生IFN-γ能力显著低于正常人及未化疗的肿瘤病人(P<0.005),这可能是由于化疗杀伤了CD_4~+、CD_(?)~+细胞。
In this study, immunofluorescence measurement of human interferon-gamma was established by using high affinity, high specificity human recombinant IFN-γ (mAb) A_3. The detection limits ranged from 0.10 to 250 U / ml.The variation coefficients within and between batches were 7.40% and 17.3%, respectively, with a recovery of 1 ± 0.17. The dilution test was linear (r = 0.98) β cross. This method was used to detect the amount of IFN-γ induced by peripheral blood mononuclear cells in 29 normal subjects, 34 tumor patients and 19 tumor chemotherapy patients. The results showed that there was no significant difference in IFN-γ production between peripheral blood mononuclear cells from tumor patients and normal persons. The ability of IFN-γ production by peripheral blood mononuclear cells in chemotherapy patients was significantly lower than that in normal and non-chemotherapy tumor patients (P <0.005) , Which may be due to chemotherapy to kill CD_4 ~ +, CD_ (~) ~ + cells.