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目的探讨Wnt/β-catenin信号通路对树突状细胞(DC)免疫学功能的调控作用。方法用RT-PCR、免疫荧光的方法鉴定Wnt通路成员在DC中的表达情况;加入活化剂SB216763激活β-catenin信号,检测其对DC表型、细胞因子及趋化因子受体表达的影响。结果 DC表达有WNT5A配体及Wnt通路多种受体Frz1、Frz2、Frz3、LRP6以及ROR2的mRNA,免疫荧光试验也证实WNT5A分布在DC的整个胞浆。SB216763能使DC胞内的β-catenin积累,并能促进DC共刺激分子CD80、CD86和CD40表达上调,但是炎性因子和趋化因子受体的mRNA并无变化甚至变低。结论 Wnt信号通路成员广泛存在于DC内,β-catenin信号活化能引起DC表型成熟,但功能上属于1种耐受型的DC。
Objective To investigate the regulatory effect of Wnt / β-catenin signaling on the immunological function of dendritic cells (DCs). Methods The expression of Wnt pathway members in DCs was identified by RT-PCR and immunofluorescence staining. Activation of SB216763 activates the expression of β-catenin and its effect on the expression of DCs, cytokines and chemokines. Results DCs expressed mRNAs of Wnt5A ligands and various receptors of Wnt pathway, such as Frz1, Frz2, Frz3, LRP6 and ROR2. Immunofluorescence assay also confirmed the distribution of WNT5A in the whole cytoplasm of DCs. SB216763 can promote the accumulation of β-catenin in DC cells and up-regulate the expression of CD costimulatory molecules CD80, CD86 and CD40, but there is no change or even lower mRNA expression of inflammatory cytokines and chemokine receptors. Conclusion The members of Wnt signaling pathway are widely present in DC. The activation of β-catenin signal causes the maturation of DC phenotype, but it is functionally a tolerant DC.