论文部分内容阅读
目的:探讨凝血因子Ⅶ(coagulation factorⅦ,FⅦ)和组织因子(tissue factor,TF)在大肠癌中的表达情况及其与临床病理因素的关系。方法:应用免疫组织化学法及Western blot对FⅦ蛋白在大肠癌组织中的表达情况进行研究;应用实时荧光定量PCR技术检测FⅦ和TF基因在45例结直肠癌患者癌及相应癌旁正常黏膜的表达。结果:(1)免疫组织化学及Western blot结果均显示FⅦ蛋白在大肠癌组织中存在异位高表达现象,癌旁正常黏膜不表达FⅦ蛋白;(2)免疫组织化学方法证实FⅦ蛋白定位表达于肿瘤细胞的胞浆和胞膜,大肠癌Ⅰ期、Ⅱ期、Ⅲ期及Ⅳ期的FⅦ表达阳性率分别为33.3%,40.0%,64.7%及80.0%(P=0.001);(3)实时荧光定量PCR方法显示大肠癌肝转移组FⅦmRNA表达明显高于非转移组,肝转移组表达量为5.33±2.88,无转移组为1.47±0.51(P=0.03),FⅦmRNA表达与肿瘤大小、分化、浸润深度、有无淋巴结转移及TNM分期无关,Spearman相关分析显示FⅦ在mRNA和蛋白水平表达呈一定的相关性,相关系数为0.58(P=0.03);(4)TF mRNA表达与大肠癌淋巴结转移、肝转移及TNM分期均有关,Logistic多因素回归分析影响肝转移的因素,结果表明TF表达是肝转移发生的重要因素(P=0.001)。结论:大肠癌组织可异源性地表达和分泌FⅦ,TF高表达是大肠癌发生肝转移的重要因素,肿瘤细胞周围高FⅦ的微环境可能促进大肠癌的转移。
Objective: To investigate the expression of coagulation factor Ⅶ (FⅦ) and tissue factor (TF) in colorectal carcinoma and its relationship with clinicopathological factors. Methods: The expression of FⅦ protein in colorectal cancer tissues was studied by immunohistochemistry and Western blot. Real-time fluorescence quantitative PCR was used to detect the expression of FⅦ and TF in 45 cases of colorectal cancer and corresponding normal mucosa expression. Results: (1) Both immunohistochemistry and Western blot showed that the FⅦ protein was highly expressed in colorectal cancer tissues, but not in the adjacent normal mucosa. (2) The localization of FⅦ protein was confirmed by immunohistochemistry The positive rates of FⅦ expression in the cytoplasm, cytoplasm of tumor cells, stage Ⅰ, Ⅱ, Ⅲ and Ⅳ of colorectal cancer were 33.3%, 40.0%, 64.7% and 80.0%, respectively (P = 0.001) Fluorescent quantitative PCR showed that the expression of FⅦ mRNA in colorectal cancer liver metastasis group was significantly higher than that in non-metastasis group (5.33 ± 2.88 vs 1.47 ± 0.51, P = 0.03). The expression of FⅦ mRNA was correlated with tumor size, differentiation, (P = 0.03). (4) The correlation between TF mRNA expression and lymph node metastasis of colorectal cancer was analyzed by Spearman correlation analysis. The expression of FⅦ mRNA and protein was correlated with the depth of invasion, lymph node metastasis and TNM stage , Liver metastasis and TNM stage. Logistic multivariate regression analysis was used to analyze the factors affecting liver metastasis. The results showed that TF expression was an important factor in liver metastasis (P = 0.001). CONCLUSION: FⅦ can be expressed and secreted heterologously in colorectal cancer. High expression of TF is an important factor in the development of liver metastasis in colorectal cancer. The high FⅦ microenvironment around tumor cells may promote the metastasis of colorectal cancer.