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目的研究花色苷矢车菊素-3-葡萄糖苷(Cy-3-G)在Caco-2细胞模型的吸收机制。方法建立Caco-2单层细胞模型,观察Cy-3-G的转运情况。加入不同浓度的维拉帕米(P-gp抑制剂)、MK571(MRP2抑制剂)、根皮苷(SGLT1抑制剂)及根皮素(GLUT2抑制剂),观察P-gp、MRP2、SGLT1及GLUT2等转运蛋白在Cy-3-G肠道吸收中的作用。结果 Cy-3-G在Caco-2细胞模型的吸收率随着时间延长逐渐升高,2 h时吸收率在0.76%~2.41%之间,但随着花色苷浓度的升高而降低。维拉帕米和MK571对Cy-3-G在Caco-2细胞模型的吸收无影响(P>0.05),根皮苷和根皮素可显著抑制Cy-3-G的吸收(P<0.05)。结论 P-gp和MRP2对Cy-3-G在肠道的吸收无影响,SGLT1和GLUT2均参与了Cy-3-G在小肠的吸收。[营养学报,2013,35(2):191-194,198]
Objective To study the mechanism of cyanidin-3-glucoside (Cy-3-G) uptake in Caco-2 cell model. Methods Caco-2 monolayer cell model was established to observe the translocation of Cy-3-G. (P-gp inhibitor), MK571 (MRP2 inhibitor), phlorizin (SGLT1 inhibitor) and phloretin (GLUT2 inhibitor) at different concentrations were added to observe the effect of P-gp, MRP2, SGLT1 and Role of GLUT2 and other transporters in intestinal absorption of Cy-3-G. Results The absorption rate of Cy-3-G in Caco-2 cell model increased gradually with the time prolonging. The absorption rate of Cy-3-G reached 0.76% -2.41% at 2 h, but decreased with the increase of anthocyanin concentration. Verapamil and MK571 had no effect on the absorption of Cy-3-G in Caco-2cells (P> 0.05). Both phlorizin and phloretin significantly inhibited the uptake of Cy-3-G (P <0.05) . Conclusion P-gp and MRP2 have no effect on the intestinal absorption of Cy-3-G, while SGLT1 and GLUT2 are involved in the absorption of Cy-3-G in the small intestine. [Journal of Nutrition, 2013,35 (2): 191-194,198]