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用伤寒沙门菌(Stw)Fe-SOD免疫家兔制备免抗Fe-SOD血清,ELISA效价为1∶10240。双向琼脂扩散试验结果显示此抗体不与牛红细胞SOD形成沉淀线,抗体可抑制Stw及鼠伤寒沙门菌(Stm)无细胞溶解物中部分SOD活性,对小鼠腹腔吞噬细胞杀灭Stw无影响(P>0.05),但对受5LD50Stm攻击的小鼠有交叉免疫保护作用(P<0.05)。
Rabbits were immunized with Salmonella typhimurium (Stw) Fe-SOD to prepare anti-Fe-SOD serum. The ELISA titer was 1:10240. Two-way agar diffusion test showed that the antibody did not precipitate with SOD of bovine erythrocytes, and the antibody could inhibit some SOD activity of Stw and Stm of cell-free lysate, and had no effect on the killing of Stw by mouse peritoneal phagocytes P> 0.05) but had a cross-protective effect on mice challenged with 5LD50Stm (P <0.05).