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比较了采用柠檬酸三钠(Trisodium citrate,Na3Ct)还原法、聚乙烯吡咯烷酮(Polyvinylpyrrolidone,PVP)包裹法和巯基丙酸-同型半胱氨酸(Mercaptopropionic Acid-Homocystine,MPA-HCys)包裹法制备的3种纳米金颗粒(Gold nanoparticles,AuNPs)对聚合酶链式反应(Polymerase Chain Reaction,PCR)扩增效率及特异性的影响,以评估金纳米材料表面性质与DNA生物大分子之间的相互作用.实验结果显示,3种AuNPs均能显著提高PCR效率,它们提高PCR效率的能力依次为:Na3 Ct-AuNPs(12 nm)>MPA-HCys-AuNPs(12 nm)>PVP-AuNPs(4 nm),而且这种增强效应与其浓度和尺寸相关.相同实验条件下,具有相似尺寸大小的Na3Ct-AuNPs比MPA-HCys-AuNPs更能有效提高PCR效率,这说明AuNPs的表面性质也是影响PCR效率的重要因素之一.另外,3种AuNPs均能有效消除竞争性引物对PCR的抑制作用,并且这种作用与其浓度密切相关.上述研究结果表明,具有不同表面性质的3种AuNPs均能有效提高PCR效率和特异性,并且这种作用与其浓度、尺寸和表面性质密切相关.
Compared with the method of Trisodium citrate (Na3Ct) reduction, polyvinylpyrrolidone (PVP) encapsulation and Mercaptopropionic Acid-Homocystine (MPA-HCys) Effect of 3 kinds of gold nanoparticles (AuNPs) on the amplification efficiency and specificity of Polymerase Chain Reaction (PCR) to evaluate the interaction between the surface properties of gold nanomaterials and DNA biomacromolecules The results showed that the three kinds of AuNPs could significantly improve the PCR efficiency and their ability to improve the PCR efficiency was as follows: Na3 Ct-AuNPs (12 nm)> MPA-HCys-AuNPs (12 nm)> PVP-AuNPs (4 nm) , And this enhancement effect was correlated with its concentration and size.Under the same experimental conditions, Na3Ct-AuNPs with similar size and size increased the efficiency of PCR more effectively than MPA-HCys-AuNPs, which indicated that the surface properties of AuNPs were also important for PCR efficiency In addition, all three kinds of AuNPs could effectively eliminate the inhibitory effect of competitive primers on PCR, and this effect was closely related to its concentration.The above results show that three kinds of AuNPs with different surface properties can have Efficiency increases the efficiency and specificity of PCR, and this effect is closely related to its concentration, size and surface properties.