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葱叶枯病菌在活体外可以产生致病毒素 ,用改良 PS培养基培养所得滤液经TLC分离获得 、 、 、 、 5种组分 ,其 Rf 值分别为 0 .31、0 .4 7、0 .74、0 .80和 0 .90 ,在乙醇中最大紫外吸收峰分别为 2 4 0、2 4 7、2 2 3、2 36和 2 58nm。生物测定结果表明 ,组分 、 、 对马唐的生长有明显的抑制作用 ,其中以组分 生物活性最高。粗毒素对禾本科杂草种子萌发抑制效果高于对阔叶杂草 ,而对玉米、水稻、油菜等种子萌发影响很小。试验还发现 ,毒素对马唐的防效最高 ,与百草枯药效相当。毒素对马唐叶绿素 a的含量影响不大 ,但对叶绿素 b和叶绿素总含量影响较大。
Onion leaf blight bacteria can produce in vitro virulence factors, with improved PS culture medium resulting filtrate was separated by TLC obtained, and, 5 kinds of components, the Rf values were 0.31,0 .4 7,0. 74,0 .80 and 0 .90, respectively. The maximum UV absorption peaks in ethanol were 240, 240, 223, 236 and 258 nm, respectively. Bioassay results show that the components, and Cynanchum growth significantly inhibited, of which the highest component bioactivity. The effect of crude toxin on the seed germination of gramineous weeds was higher than that of broadleaf weeds, but little effect on the seed germination of maize, rice and rapeseed. The test also found that toxins on the Cangban the highest control effect, and paraquat efficacy quite. Toxin had little effect on the content of chlorophyll a, but had a great effect on the total content of chlorophyll b and chlorophyll.