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目的:观察葛根素对兔肺缺血再灌注损伤中细胞凋亡的影响。方法:复制兔单侧肺缺血再灌注损伤模型,随机分为三组,每组10只:对照组(C组)、缺血再灌注组(IR组)和葛根素组(Pur组)。对比观察各组血浆超氧化物歧化酶(SOD)活力、一氧化氮(NO)及丙二醛(MDA)含量、肺湿干重比(W/D)、肺泡损伤数定量评价指标(IQA)及肺组织细胞凋亡指数(AI)。结果:再灌注后各时间点IR组与C组比较发现,SOD活力、NO含量明显降低(P<0.01),MDA、W/D、IQA、AI明显升高(P<0.01),Pur组与IR组相比较发现,SOD活力、NO含量均明显升高(P<0.01),MDA、W/D、IQA、AI不同程度地有所降低(P<0.05)。结论:葛根素通过抗氧化与提高NO含量,可抑制再灌注后肺组织细胞凋亡,从而减轻肺损伤。
Objective: To observe the effect of puerarin on apoptosis of rabbit lung ischemia-reperfusion injury. METHODS: Rabbit models of unilateral lung ischemia-reperfusion injury were duplicated and randomly divided into three groups of 10 rats: control group (C), ischemia-reperfusion group (IR group) and puerarin group (Pur group). Observe the superoxide dismutase (SOD) activity, nitric oxide (NO) and malondialdehyde (MDA) content, the wet/dry weight ratio (W/D) of the lung, quantitative assessment index of alveolar damage (IQA) in each group. And lung tissue apoptosis index (AI). Results:Compared with C group at each time point after reperfusion, the SOD activity and NO content were significantly decreased (P<0.01), MDA, W/D, IQA and AI were significantly increased (P<0.01). Compared with IR group, SOD activity and NO content were significantly increased (P<0.01), while MDA, W/D, IQA, and AI were decreased to some extent (P<0.05). Conclusion: Puerarin can inhibit the apoptosis of lung tissue after reperfusion and reduce the lung injury through anti-oxidation and increase of NO content.