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目的探讨TRAIL选择性诱导肿瘤细胞凋亡,而不损伤正常组织的可能机制。方法采用免疫组织化学SP法,检测38例直肠癌及其癌旁组织,14例正常直肠黏膜组织中TRAIL及DcR1、DR4受体、caspase3、bcl-2在直肠癌及癌旁组织中的蛋白表达情况,结合其临床病理学资料,分析它们之间的相关性。结果TRAIL、DcR1、DR4、caspase3与bcl-2在正常直肠黏膜、癌旁6cm组织、癌组织上皮中阳性表达有显著性差异(P<0.05),其中TRAIL、DcR1、DR4、caspase3、bcl-2癌组织上皮与正常直肠黏膜阳性表达均有显著性差异(P<0.05),TRAIL、DcR1、DR4、bcl-2在癌组织上皮与癌旁6cm组织上皮中的表达有显著性差异(P<0.05);TRAIL、DcR1、DR4、caspase3与bcl-2在正常直肠黏膜与癌旁6cm组织上皮中的表达均无显著性差异(P>0.05);TRAIL与bcl-2高中分化癌组织中与低分化癌组织中的表达均有显著性差异(P<0.05);DcR1、DR4、caspase3与肿瘤分化程度无关;TRAIL、DcR1、DR4、caspase3与bcl-2在癌组织中的表达与性别、年龄、分期、肿瘤位置、肿瘤大小及组织类型无关(P>0.05)。结论DcR1、DR4、caspase3、bcl-2与TRAIL作用机制相关,其中DcR1与bcl-2可能是抑制因素,而DR4、caspase3是TRAIL作用环节的关键指标。
Objective To explore the possible mechanism of TRAIL selectively inducing tumor cell apoptosis without damaging normal tissues. Methods The expressions of TRAIL, DcR1, DR4 receptor, caspase3 and bcl-2 in rectal cancer tissues and paracancer tissues were detected by immunohistochemical SP method in 38 cases of rectal cancer and adjacent tissues and 14 cases of normal rectal mucosa. Cases, combined with its clinical pathology data, analyze the correlation between them. Results The positive expressions of TRAIL, DcR1, DR4, caspase3 and bcl-2 in normal rectal mucosa, paracancer 6cm and cancer tissue epithelium were significantly different (P <0.05) The expression of TRAIL, DcR1, DR4 and bcl-2 were significantly different between the epithelium of the cancer tissue and the adjacent epithelium of 6cm (P <0.05) ). There was no significant difference in the expression of TRAIL, DcR1, DR4, caspase3 and bcl-2 in normal rectal mucosa and adjacent 6cm tissue epithelium (P> 0.05) The expressions of TRAIL, DcR1, DR4, caspase3 and bcl-2 in cancer tissues were significantly different from those in cancer tissues (P <0.05). The expressions of DcR1, DR4 and caspase3 were not correlated with the degree of tumor differentiation. , Tumor location, tumor size and type of tissue (P> 0.05). Conclusion DcR1, DR4, caspase3 and bcl-2 are related to the mechanism of TRAIL. DcR1 and bcl-2 may be the inhibitory factors, while DR4 and caspase3 are the key indicators of TRAIL.