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目的探讨超声无创评价大鼠子宫腺肌病建模成功的可行性。方法 18只成熟未孕雌性Wistar大鼠随机分为建模组12只和对照组6只,建模组用自体内膜种植法手术建模,对照组同法手术但不种植内膜。术前及术后1、2个月超声和大体标本测量的子宫体厚度测值进行比较分析,最后镜下观察术后子宫肌层内膜腺体浸润情况。结果术前常规超声较难显示大鼠子宫,需借助阴道置管超声实时动态寻找子宫,建模组术后1、2个月常规超声较易显示出子宫形态(为条状),超声和大体标本测量的子宫体厚度较术前明显增加,二者间比较差异无统计学意义(P>0.05),二者测值有很好相关性,呈正相关关系(r分别为0.943 3、0.968 9、0.868 6,P<0.05);术后2个月测值较术后1个月增加,差异有统计学意义(P<0.05);镜下观察术后1、2个月肌层内均可见典型的内膜腺体。对照组术后同期超声和大体标本测值较术前无明显变化,镜下观察肌层内均无内膜腺体浸润。结论超声测量子宫体厚度可作为判定大鼠子宫腺肌病建模是否成功的无创手段。
Objective To investigate the feasibility of noninvasive ultrasound-based evaluation of modeling of adenomyosis in rats. Methods Twenty-eight mature and non-pregnant female Wistar rats were randomly divided into model group (n = 12) and control group (n = 6). The model group was established by autologous endomembrane surgery. Preoperative and postoperative 1 month and 2 months after ultrasound and gross specimen measurement of uterine thickness measured comparative analysis, the final microscopic observation of myometrium intimal gland infiltration. Results Conventional ultrasound was more difficult to show the uterus in rats. The vaginal catheterization was needed to find out the uterus in real time. Conventional ultrasound in the modeling group was more likely to show uterus morphology (strip), ultrasound and gross The thickness of the uterine body measured by the specimen increased significantly compared with the preoperative value, with no significant difference between the two groups (P> 0.05). There was a positive correlation between the two measurements (r = 0.943 3,0.968 9, 0.868 6, P <0.05). The measured values at 2 months after operation increased more than 1 month after operation, with significant difference (P <0.05) Of the endometrial glands. In the control group, there was no significant change in the ultrasound and gross specimen in the same period after operation compared with that before operation. There was no endometrial gland infiltration in the muscle layer in the control group. Conclusion Ultrasound measurement of uterine body thickness can be used as a noninvasive means to determine the success of modeling adenomyosis in rats.