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S_(180)-V 系细胞,是通过悬浮静置培养过渡到单层静置培养而建成的。至今年6月已传至一百代。该系细胞形状不一,核大,常见单核或多核巨细胞。体外生长的细胞,约60%贴瓶生长,40%悬浮生长。悬浮生长的细胞,换瓶后仍能贴瓶生长。细胞群体倍增时间为14—20小时。分裂指数平均为35‰,分裂期的时长平均1.2小时。饱和密度约24×10~4/平方厘米。染色体众数值为88,具中双着丝点标记染色体。以10~6细胞/鼠的细胞数接种于小鼠,全部动物长成实体瘤或腹水瘤。
The S_ (180) -V lineage cells were established by resting static culture in suspension to single-layer static culture. It has been passed to one hundred generations in June this year. The Department of cell shapes, large nuclear, common mononuclear or multinucleated giant cells. In vitro growth of cells, about 60% paste bottle growth, 40% suspended growth. Floating growth of cells, bottles can still bottle after bottle growth. Cell population doubling time is 14-20 hours. The average index of division was 35 ‰, and the duration of division was 1.2 hours on average. Saturated density of about 24 × 10 ~ 4 / square centimeter. Chromosome number 88, with double-centromere markers chromosome. Mice were inoculated with 10-6 cells / mouse cells, all animals grew into solid tumors or ascites tumors.