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目的观察实验性肝纤维化过程中间质胶原酶（MMP－13）及其组织金属蛋白酶抑制因子-1（TIMP－1）基因表达的不平衡性。方法建立CCl4中毒性大鼠肝纤维化模型，采用SABC免疫组化方法和逆转录定量PCR方法测定MMP-13和TIMP－1的表达情况。结果MMP-13和TIMP－1在正常大鼠肝组织中有表达，在肝纤维化发生发展过程中MMP-13表达无显著性变化，而TIMP－1的表达则逐渐增强，在肝硬化阶段达到最高值。结论MMP-13和TIMP-1在肝纤维化过程中的不平衡表达可能是肝硬化形成的重要决定因素。
Objective To investigate the imbalance of the expression of matrix metalloproteinase-1 (MMP-13) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in experimental liver fibrosis. Methods CCl4-induced rat liver fibrosis model was established. The expressions of MMP-13 and TIMP-1 were detected by SABC immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR). Results MMP-13 and TIMP-1 were expressed in the normal rat liver tissue. There was no significant change in the expression of MMP-13 during the development of hepatic fibrosis, while the expression of TIMP-1 gradually increased in the stage of liver cirrhosis The highest value. Conclusion The imbalanced expression of MMP-13 and TIMP-1 in liver fibrosis may be an important determinant of cirrhosis.