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目的从中国南海花柳珊瑚来源真菌Aspergillus candidus(RA16-10)中分离鉴定具有生物活性的代谢产物。方法运用硅胶柱层析、Sephadex LH-20凝胶柱层析和半制备HPLC等方法分离并分析化合物,运用核磁、质谱等现代波谱分析方法对化合物进行结构鉴定,并对化合物进行抗菌、卤虫致死、藤壶幼虫致死等活性评价。结果从A.candidus中分离鉴定了3个黄酮类化合物(1~3)和1个三联苯类化合物(4),其中含有氯原子的黄酮类化合物1和3是自然界中极其罕见的化合物;化合物1和2显示出强的藤壶幼虫Balanua amphitrite致死活性,其LC_(50)值分别为1.11μg/mL和1.39μg/mL,这是首次报道该类化合物的藤壶幼虫致死活性。结论从中国南海花柳珊瑚来源真菌A.candidus(RA16-10)中筛选发现了2个具有显著的藤壶幼虫B.amphitrite致死活性的黄酮类化合物,这类化合物具有开发成为防污剂的潜力。
OBJECTIVE To isolate and identify bioactive metabolites from Aspergillus candidus (RA16-10) from the genus Coralis in the South China Sea. Methods The compounds were separated and identified by silica gel column chromatography, Sephadex LH-20 gel column chromatography and semi-preparative HPLC. The structures of the compounds were identified by using modern spectroscopic methods such as nuclear magnetic resonance and mass spectrometry. Lethal, barnacle larvae lethal activity evaluation. Results Three flavonoids (1-3) and one terphenyl compound (4) were isolated and identified from A. garidus. Flavonoids 1 and 3 containing chlorine atoms were the most rare compounds in nature. Compounds 1 and 2 showed strong lethal activity of Balanua amphitrite in barnacle larvae with LC_ (50) values of 1.11μg / mL and 1.39μg / mL, respectively. This is the first report of the killing activity of barnacle larvae of these compounds. Conclusion Two flavonoids with significant lethal activity of B. camphitrite from Larvae corallinate fungi A.candidus (RA16-10) in the South China Sea were found. These compounds have the potential to develop into antifouling agents.