本文分两部分。第一部分简单地介绍了非催化动力学分析法,指出速差动力学分析法对性质相似混合物测定的实用价值,推导了基本方程式及对数外推法处理。第二部分介绍了酶法分析的米氏方程式,指出本法对生命科学的意义,并用实例阐述了在酶活性、底物、活化剂和阻抑制在测定中的应用。 This article is divided into two parts. In the first part, the non-catalytic kinetic analysis method is briefly introduced. The practical value of differential kinetic analysis for the determination of similar mixtures is pointed out. The basic equations and the logarithm extrapolation method are derived. The second part introduces the Michaelis-Menten equation of enzymatic analysis, points out the significance of this law to life science, and expounds the application of enzyme activity, substrate, activator and inhibition in the determination with examples.