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目的探讨非小细胞肺癌(NSCLC)组织和患者外周血中CD40的表达及其临床意义。方法采用免疫组织化学和酶联免疫吸附试验(ELISA)等方法检测经胸外科手术切除的60例NSCLC组织、15例癌旁肺组织和患者外周血中CD40的表达情况。结果 (1)NSCLC组织中CD40阳性表达率较癌旁肺组织明显增加(P<0.05);(2)有淋巴结转移组的CD40阳性表达率明显高于无淋巴结转移组(P<0.05)。Ⅲ～Ⅳ期NSCLC组织CD40阳性表达率明显高于Ⅰ～Ⅱ期(P<0.05)。(3)NSCLC外周血清可溶性CD40(sCD40)的表达水平明显高于健康对照组(P<0.05);(4)NSCLC患者外周血清sCD40含量和NSCLC组织中CD40分子表达成正相关关系(P<0.05)。结论 NSCLC患者体内CD40含量增加可增强肺癌的侵袭和免疫逃逸能力;患者外周血清sCD40含量升高可能和肺癌细胞表面CD40分子释放有关。
Objective To investigate the expression of CD40 in peripheral blood of patients with non-small cell lung cancer (NSCLC) and its clinical significance. Methods Immunohistochemistry and enzyme-linked immunosorbent assay (ELISA) were used to detect the expression of CD40 in peripheral blood of 60 cases of NSCLC and 15 cases of paracancerous lung tissue removed by thoracic surgery. Results (1) The positive expression rate of CD40 in NSCLC tissues was significantly higher than that in paracancer lung tissues (P <0.05). (2) The positive rate of CD40 expression in lymph node metastasis group was significantly higher than that in non-lymph node metastasis group (P <0.05). The positive expression rate of CD40 in stage Ⅲ ~ Ⅳ NSCLC was significantly higher than that in stage Ⅰ ~ Ⅱ (P <0.05). (3) The expression of soluble CD40 (sCD40) in peripheral blood of NSCLC patients was significantly higher than that of healthy controls (P <0.05). (4) The positive correlation between the level of sCD40 in peripheral blood and the expression of CD40 in NSCLC was significant . Conclusion The increase of CD40 content in NSCLC patients can enhance the invasion and immune escape ability of lung cancer. The increased sCD40 content in peripheral blood serum of patients with NSCLC may be related to the release of CD40 on the surface of lung cancer cells.