目的观察在不同的时机应用利尿酸以暂时破坏耳蜗血管纹上皮是否能够开放血-迷路屏障,从而促使庆大霉素进入耳蜗或者从耳蜗排出。方法听神经动作电位测试技术,全耳蜗毛细胞计数定量观察技术和荧光偏振免疫法测定庆大霉素浓度的技术被用于以下两个不同目的的实验观察。(1)当庆大霉素血中浓度高于内耳液浓度时,应用利尿酸破坏蜗管外壁以促使更多的庆大霉素进入耳蜗以制备不同程度耳蜗损害的动物模型。(2)当庆大霉素内耳浓度高于血中浓度时,应用利尿酸损坏蜗管外壁以促使蓄积在耳蜗内的庆大霉素从内耳排出以达到挽救毛细胞的目的。结果1郾当庆大霉素血中浓度高于内耳液浓度时,注射利尿酸可造成更多的毛细胞损害和听功能障碍,外淋巴中药物的峰值浓度和半衰期浓度也均比单独一次注射庆大霉素动物外淋巴中药物浓度增加一倍,说明同时注射利尿酸可促使更多的庆大霉素从血液进入耳蜗并造成更严重的毛细胞损害。2郾当血液中庆大霉素排空之后,注射利尿酸可减少毛细胞数量的损失程度,同时发现延迟注射利尿酸组动物的听力损失程度比不经利尿酸处理动物组有所减轻,外淋巴中药物浓度也比不经利尿酸处理动物组降低一半,说明当GM在耳蜗内蓄积但血清中已经排空时注射EA有助于降低药物在耳蜗内的蓄积并挽救尚未被破坏的毛细? Objective To observe whether the application of diuretic at different times to temporarily disrupt the cochlear stria vascularis can open the blood-labyrinth barrier, thereby promoting the entry of gentamicin into the cochlea or excretion from the cochlea. Methods The technique of auditory nerve action potential test, total cochlear hair cell count quantitative observation technique, and fluorescence polarization immunoassay for the determination of gentamicin concentration were used for the following two experimental observations of different purposes. (1) When the concentration of gentamycin in the blood is higher than that of the inner ear fluid, uric acid is used to destroy the outer wall of the scrolls to induce more gentamicin into the cochlea to prepare animal models of varying degrees of cochlear damage. (2) When the concentration of gentamicin in the inner ear is higher than the concentration in the blood, uric acid is used to damage the outer wall of the sclerotiorum to promote the release of gentamicin accumulated in the cochlea from the inner ear in order to save the hair cells. Results When the concentration of gentamycin in the blood is higher than that of the inner ear fluid, injection of uric acid can cause more hair cell damage and hearing dysfunction, and the peak concentration and half-life concentration of the drug in the perilymph are also higher than those in the single injection Gentamycin doubled the concentration of the drug in the perilymph, suggesting that simultaneous injection of diuretic may promote more gentamicin from the bloodstream into the cochlea and cause more severe hair cell damage. 2 郾 When the blood of gentamicin after emptying, injection of uric acid can reduce the loss of hair cell number, also found that delayed injection of uric acid group of animals, the degree of hearing loss than the group without the treatment of uric acid to reduce, outside The drug concentration in lymphatic vessels was also reduced by half compared with the group without the treatment of uric acid, indicating that injection of EA can reduce the accumulation of drugs in the cochlea and save the undisturbed capillary when GM accumulates in the cochlea but the serum has been emptied.