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目的:探讨定经汤调节卵巢早衰的生物学机制。方法:SD大鼠90只,随机分为3组:正常组、模型组、定经汤干预组;分别检测其与卵巢早衰相关的生物分子:β-内啡肽(β-EP)、白介素-1(IL-1)、一氧化氮合酶(NOS)、一氧化氮(NO)、雌激素受体(ER)、孕激素受体(PR)、促性腺激素释放激素(GnRH)、促肾上腺皮质激素释放激素(CRH)、促卵泡激素(FSH)、黄体生成素(LH)、雌二醇(E2)、孕酮(P)、促肾上腺皮质激素(ACTH)和皮质醇(CORT);利用因子分析方法探究定经汤干预下各生物分子之间的相互调控关系,并探寻定经汤调节卵巢早衰过程中的关键生物分子。结果:定经汤干预状态的生殖内分泌网络主调控因子主要包括垂体-卵巢轴上的4个生物分子,垂体-肾上腺轴靶腺层生物分子CORT以及部分下丘脑生物分子;IL-1是定经汤干预状态生殖内分泌网络主调控因子与其他公共因子共有的下丘脑部位的生物分子。结论:定经汤一方面可以直接调节垂体-肾上腺轴生物分子,另一方面又可以通过下丘脑间接调节垂体-肾上腺轴生物分子;各调控因子之间起枢纽作用的生物分子为IL-1。
Objective: To investigate the biological mechanism of Dingjing Decoction in regulating premature ovarian failure. Methods: Ninety Sprague-Dawley rats were randomly divided into three groups: normal group, model group and Dingjing Decoction group. The biological molecules related to premature ovarian failure were detected: β-EP, 1, IL-1, NOS, NO, ER, PR, GnRH, adrenal gland (CRH), follicle stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2), progesterone (P), adrenocorticotropic hormone (ACTH) and cortisol (CORT) The factor analysis method explored the mutual regulation of the biomolecules under the action of Dingjing Decoction, and explored the key biomolecules in the process of decoction of premature ovarian failure by Dingjing Decoction. Results: The main regulatory factors of the reproductive and endocrine network of Dingjing Decoction include four biomolecules on the pituitary-ovary axis, the target gland biotin CORT and some hypothalamic biomolecules in the pituitary-adrenal axis; IL-1 Soup Interventions State of the Reproductive Endocrine Network Main Regulators Biotypes of Hypothalamic Parts Common to Other Common Factors. Conclusion: Dingjing Decoction can directly regulate the pituitary-adrenal axis biomolecules and indirectly regulate the pituitary-adrenal axis biomolecules through the hypothalamus. The biomolecules that play a pivotal role among various regulators are IL-1.