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目的评价亚硒酸钠(Na2SeO3)的致突变性和抗突变性。方法使用体外和体内微核试验。体外试验设阴性对照组、阳性对照组、抗突变对照组,以及致突变和抗突变试验各5组,处理L5178Y细胞3 h、低渗、固定、制片。体内试验设阴性对照组、阳性对照组,以及致突变和抗突变试验各4组,昆明种小鼠连续灌胃5 d,末次灌胃后24 h处死小鼠,取骨髓制片,染色,观察。结果体外试验中,3～24μg/ml Na2SeO3致突变试验组的细胞微核率与阴性对照组相比差异有统计学意义,0.2～3.2μg/ml Na2SeO3抗突变试验组的细胞微核率均比丝裂霉素C(MMC)对照组低,差异有统计学意义;体内微核试验中,6.32、7.90 mg/kg.bw剂量组的小鼠骨髓细胞微核率与阴性对照组相比差异有统计学意义,0.04～0.32 mg/kg.bw剂量组的微核率明显低于环磷酰胺(CP)对照组,差异有统计学意义。结论亚硒酸钠在一定的剂量范围内表现出致突变作用,而在不同的剂量范围内又可拮抗CP和MMC引起的遗传损伤。
Objective To evaluate the mutagenicity and anti-mutagenicity of sodium selenite (Na2SeO3). Methods Using in vitro and in vivo micronucleus assays. In vitro experiments were set negative control group, positive control group, anti-mutation control group, mutagenesis and anti-mutation test in 5 groups, L5178Y cells were treated 3 h, hypotonic, fixed, filming. In vivo, negative control group, positive control group, mutagenicity and anti-mutation test were conducted in 4 groups. Kunming mice were given gavage for 5 consecutive days, and mice were killed 24 h after the last gavage. . Results In vitro, the micronucleus rate of 3 ~ 24μg / ml Na2SeO3 mutagenicity test group was significantly different from that of the negative control group. The micronucleus rate of 0.2 ~ 3.2μg / ml Na2SeO3 anti-mutation test group was Mitomycin C (MMC) control group, the difference was statistically significant; in vivo micronucleus test, 6.32,7.90 mg / kg.bw dose group of mouse bone marrow micronucleus rate compared with the negative control group, there are differences Statistical significance, micronucleus rate of 0.04 ~ 0.32 mg / kg.bw dose group was significantly lower than cyclophosphamide (CP) control group, the difference was statistically significant. Conclusion Sodium selenite shows a mutagenic effect in a certain dose range, and can antagonize the genetic damage caused by CP and MMC in different dose ranges.