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目的观察原花青素(PC)对氨基脲(SEM)致雄性小鼠生殖毒性的拮抗作用及作用机制。方法 50只KM雄性小鼠随机等分为5组:溶剂对照组、SEM染毒组、低、中、高剂量PC保护组。溶剂对照组灌胃去离子水;SEM染毒组先按56.25 mg/kg.bw SEM溶液灌胃染毒2周,再继续灌胃去离子水4周;3个剂量PC保护组,先分别灌胃PC 100、200、400 mg/kg.bw 4周,继续分别灌胃同剂量SEM溶液2周;以上各组动物均按10 ml/kg.bw体积灌胃每日1次,连续灌胃6周剖杀,取其血液和组织器官检测生殖相关指标。结果 PC保护组睾丸形态较SEM染毒组有所改善,且随PC剂量的加大改善较明显;低、中、高剂量PC组小鼠精子数、精子活动度较SEM组增加,畸形率降低;血清T、LH、FSH含量均高于SEM染毒组,SOD、GSH-PX活力上升、MDA、GSH含量下降与SEM染毒组比较,差异均有统计学意义(P<0.05)。结论适量PC可保护SEM对雄性小鼠亚慢性生殖系统的损伤;抗氧化损伤是PC保护SEM致生殖损伤的重要机制。
Objective To observe the antagonistic effect and mechanism of proanthocyanidins (PC) on reproductive toxicity induced by semicarbazide (SEM) in male mice. Methods Fifty KM male mice were randomly divided into five groups: solvent control group, SEM exposure group, low, medium and high dose PC protection group. The solvent control group was given gavage with deionized water. SEM exposure group was treated with 56.25 mg / kg. Bw SEM solution for 2 weeks and then deionized water for 4 weeks. Three doses of PC protection group Stomach PC 100,200,400 mg / kg.bw for 4 weeks, respectively, continued to intragastric administration of the same dose of SEM solution for 2 weeks; the above groups of animals were fed by 10 ml / kg.bw volume once daily, continuous gavage 6 Week profile, take the blood and tissues and organs detection of reproductive related indicators. Results Compared with the SEM group, the morphology of testis in PC group was improved and the improvement was more obvious with the increase of PC dose. The sperm count and sperm motility in PC group increased and the deformity rate decreased (P <0.05). The levels of serum T, LH and FSH in SEM group were higher than that in SEM group. The activities of SOD and GSH-PX increased, while the contents of MDA and GSH decreased in SEM group. Conclusion The moderate amount of PC protects the subchronic reproductive system of male mice by SEM. Antioxidant injury is an important mechanism of protecting PC induced reproductive injury by PC.