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目的采用蛋白芯片法对铅中毒儿童的血浆蛋白进行筛查,以寻找并筛选与铅神经毒性机制相关的信号蛋白,为研究铅神经毒性提供新的生物学标志物。方法选取2014年1-12月进行健康体检的1~7岁儿童190例,包括铅中毒儿童28例(高血铅组)及低血铅儿童162例(低血铅组),收集其静脉血标本并离心,-80℃保存血浆。应用蛋白芯片技术对4例铅中毒和4例低血铅儿童的血浆蛋白进行筛查,运用ELISA法对纳入的其他儿童的血浆特异蛋白进行验证。结果在筛查阶段,蛋白芯片技术发现IL-8为差异蛋白;在验证阶段,ELISA法发现高血铅组儿童的血浆IL-8水平(中位数=4 296.38 pg/ml,P_(25)~P_(75)=3 145.35~4 804.92 pg/ml)明显高于低血铅组(中位数=1 675.24 pg/ml,P_(75)~P_(25)=1 457.85~2 508.59 pg/ml,t=128.31,P=0.000)。结论 IL-8为血浆差异蛋白,这可能与铅神经毒性的分子病理机制有关,为铅神经毒性的研究提供了新的生物学标志物。
Objective To screen the plasma proteins of children with lead poisoning by protein chip method to find and screen the signal proteins related to lead neurotoxicity and to provide new biomarkers for the study of lead neurotoxicity. Methods Totally 190 children aged 1 ~ 7 years old, including 28 children with lead poisoning (high blood lead group) and 162 children with low blood lead (low blood lead group), were enrolled in this study. The venous blood Specimens were centrifuged and stored at -80 ° C. Plasma proteins of 4 children with lead poisoning and 4 children with low blood lead were screened by protein chip technology and the plasma specific proteins of other children were verified by ELISA. Results At the screening stage, IL-8 was found to be a differential protein in the protein chip assay. Plasma IL-8 levels in children with hyperleukocytosis were found by ELISA in the validation phase (median = 4 296.38 pg / ml, P 25) P 75 75 3 5 4 804.92 pg / ml was significantly higher than that of the low blood lead group (median = 1675.24 pg / ml, P 75- P 25 = 1 457.85-2 508.59 pg / ml, t = 128.31, P = 0.000). Conclusion IL-8 is a plasma differential protein, which may be related to the molecular pathological mechanism of lead neurotoxicity, providing a new biomarker for the study of lead neurotoxicity.