本文报告免疫刺激结合化学治疗对杜氏利什曼无鞭毛期的杀灭作用。体外试验取雌性C57B1/6鼠的腹腔巨噬细胞与从杜氏利什曼感染鼠的脾组织中获得的无鞭毛体共同培养。无鞭毛体与巨噬细胞比率为10∶1～20∶1,用光学显微镜计算巨噬细胞中含完整无鞭毛体的数量来评价效果。共试验了3种巨噬细胞,即腹腔注射硫甘醇酸钠刺激产生的、从H_(87)R_a株结核杆菌慢性感染的小鼠腹腔中 This article reports the immunostimulatory and immunotherapy combined with chemotherapy to kill Leishman’s non-flagellum period. In Vitro Assay Peritoneal macrophages from female C57B1 / 6 mice were co-cultured with amastigotes obtained from spleen tissue of infected mice. The ratio of amastigotes to macrophages was 10: 1 to 20: 1 and the number of intact amastigotes in macrophages was counted by light microscopy to evaluate the effect. A total of three macrophages were tested, ie intraperitoneally injected with sodium thioglycollate, from the peritoneal cavity of chronic infection of Mycobacterium tuberculosis H_ (87) R_a strain