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为筛选朱砂七提取物体外抑制9株临床分离致病菌和抑制新城疫病毒(NDV)的活性部位,采用琼脂扩散和试管稀释法进行体外抑菌试验,MTT法和鸡胚法测定了朱砂七提取物对NDV在鸡胚成纤维细胞(CEF)和鸡胚内增殖的影响。结果显示,朱砂七乙酸乙酯部位(PCE)对9株临床分离菌的MBC均小于100 g/L;朱砂七氯仿部位(PCC)有一定的抑菌作用;大黄素对沙门菌(鸡)、链球菌(猪)、无乳链球菌(奶牛)、表皮葡萄球菌(奶牛)和金黄色葡萄球菌(奶牛)的MBC分别为5,2.5,2.5,2.5,1.25 g/L;PCE进一步分离部位PCEA对沙门菌(鸡)、链球菌(猪)、无乳链球菌(奶牛)、表皮葡萄球菌(奶牛)和金黄色葡萄球菌的MBC分别为24,24,12,6,6 g/L。PCC和大黄素对NDV在CEF上增殖的抑制作用不明显;PCE对NDV在CEF上的抑制率为39.12%(P<0.01),PCEA对NDV在CEF上的抑制率为72.33%(P<0.01);PCEA对NDV在鸡胚中增殖具有抑制作用,其预防组和药毒混合组与病毒对照组的血凝效价差异显著(P<0.05),而治疗组与病毒对照组差异不显著。由此可知,朱砂七提取物具有抑制细菌作用,PCE和PCEA对NDV的增殖具有一定的抑制作用。
In order to screen the extracts of cinnabar seven in vitro to inhibit the activity of 9 clinically isolated pathogens and to inhibit the activity of Newcastle disease virus (NDV), agar diffusion and in vitro antibacterial test were conducted. MTT assay and chicken embryo assay Effects of Extracts on Proliferation of NDV in Chick Embryonic Fibroblasts (CEFs) and Chickens. The results showed that the MBC of ethyl acetate fraction (PCE) of cinnabar to 9 clinical isolates was less than 100 g / L, and the PCC of cinnabar had certain antibacterial activity. The inhibitory effect of emodin on Salmonella, The MBC of Streptococcus (pig), Streptococcus agalactiae (Dairy cow), Staphylococcus epidermidis (Dairy cow) and Staphylococcus aureus (Dairy cow) were 5,2.5,2.5,2.5,1.25 g / L, respectively; The MBC for Salmonella (chicken), Streptococcus (pig), Streptococcus agalactiae (cows), Staphylococcus epidermidis (cows) and Staphylococcus aureus were 24, 24, 12, 6, 6 g / L, respectively. The inhibitory effect of PCE and emodin on the proliferation of NDV was not obvious. The inhibition rate of NDV on CEF by PCE was 39.12% (P <0.01), and that of PCEA on NDV was 72.33% (P <0.01) ). PCEA inhibited the proliferation of NDV in chicken embryos. The titer of hemagglutination was significantly higher in the prophylaxis group, the drug-drug combination group and the virus control group (P <0.05), while the difference between the treatment group and the virus control group was not significant. It can be seen, cinnabarinus extract has a bacteriostatic effect, PCE and PCEA on the proliferation of NDV has some inhibitory effect.