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利用PMSG(妊娠母马血清促性腺激素)及HCG(人羢膜促性腺激素)处理的未成年大鼠卵巢匀浆,通过测定Δ~5-3β-羟类固醇脱氢酶(HSD)活性,研究了酪氨酸影响孕酮的代谢机理。结果表明,同一浓度的酪氨酸(60微克/毫升)加到排卵后不同时间(12—72小时)的卵巢匀浆中,Δ~5-3β-HSD活性增加,并随着时间延长有逐渐增加的趋势。酪氨酸组同对照组间差异非常显著(P<0.001),不同浓度的酪氨酸(30—160微克/毫升)加到排卵后同一时间(24小时)的卵巢匀浆,也能提高Δ~5-3β-HSD活性,酪氨酸组与对照组间差异非常显著(P<0.001)。
By measuring the activity of Δ5-3β-hydroxysteroid dehydrogenase (HSD) in ovary homogenates of immature rats treated with PMSG (gestational mare serum gonadotropin) and HCG (human chorionic gonadotropin) Tyrosine influences the metabolism of progesterone. The results showed that the same concentration of tyrosine (60 μg / ml) was added to ovarian homogenates at different times (12-72 hours) after ovulation, Δ5-3β-HSD activity increased, and gradually increased over time Increasing trend. The difference between the tyrosine group and the control group was significant (P <0.001). Addition of different concentrations of tyrosine (30-160 μg / ml) to ovarian homogenates at the same time after ovulation (24 hours) also increased Δ ~ 5-3β-HSD activity, the tyrosine group and the control group difference was significant (P <0.001).