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以初始质量为(33.52±0.17)g建鲤鱼种为研究对象,在室内单循环养殖系统中进行8周(w)生长试验,分别配制添加0.0%(对照饲料)和0.5%(试验饲料)丙氨酰-谷氨酰胺(Ala-Gln)的等氮(35 g/kg粗蛋白)、等能(17kJ/g能量)饲料,采用5种不同的Ala-Gln投喂方式[连续8w投喂对照饲料(I,对照组);试验饲料2w交替投喂(II);前4w投喂试验饲料、后4w投喂对照饲料(III);前4w投喂对照饲料、后4w投喂试验饲料(IV);8w连续投喂试验饲料(V)],探讨Ala-Gln投喂方式对建鲤生长、抗氧化及免疫力的影响。结果表明:Ala-Gln连续投喂和不连续投喂的生长都显著高于对照组(P<0.05)。2w交替投喂的生长率显著高于4w交替和连续8w投喂试验饲料组(P<0.05),前4w投喂试验饲料且后4w投喂对照饲料的生长率要高于8w连续投喂试验饲料组(P<0.05)。后4w投喂试验饲料组和连续8w投喂试验饲料组的血清SOD显著高于对照组(P<0.05);连续8w投喂试验饲料组显著高于2w交替投喂试验饲料组和前4w投喂试验饲料组的血清SOD(P<0.05)。Ala-Gln各种投喂方式组的肝胰脏谷胱甘肽过氧化物酶(GSH-Px)都显著高于对照组(P<0.05)。后4w投喂试验饲料组和连续8w投喂试验饲料组的血清GSH-Px显著高于对照组(P<0.05)。在Ala-Gln四种投喂方式组中,除2w交替投喂组外,其他三种投喂方式组的头肾LZM都显著高于对照组(P<0.05)。后4w投喂试验饲料组和8w连续投喂试验饲料组的脾脏LZM都显著高于对照组(P<0.05)。对照组头肾NO要显著高于各投喂方式组(P<0.05)。在试验条件下,饲料中添加Ala-Gln可提高建鲤的生长、抗氧化和免疫力。不同投喂方式间亦有显著差异,从生长、抗氧化和免疫的角度,结合经济性和适用性等进行考虑,建议采用2w间隔投喂的方式。
The carp species with the initial mass of (33.52 ± 0.17) g were studied for 8 weeks (w) growth experiment in a single-cycle indoor culture system, and were respectively formulated with 0.0% (control feed) and 0.5% (test feed) Equivalent (17 kJ / g energy) diet was fed with equal nitrogen (35 g / kg crude protein) of Ala-Gln and 5 different Ala-Gln diets (I, control group). The experimental diets were fed for 2 weeks alternately (II). The experimental diets were fed 4 weeks before the control diets (III) and the control diets (IV) ); 8w continuous feeding of test feed (V)] to investigate the effects of Ala-Gln feeding on the growth, antioxidation and immunity of Jian carp. The results showed that the growth of Ala-Gln was significantly higher than that of the control group (P <0.05). 2w alternately fed the growth rate was significantly higher than the 4w alternating and continuous 8w feeding experimental feed group (P <0.05), the first 4w fed the experimental feed and 4w after feeding the control feed growth rate was higher than the 8w continuous feeding test Feed group (P <0.05). After 4 weeks of feeding, the serum SOD of the experimental diets fed with the experimental diets was significantly higher than that of the control group (P <0.05). The diets fed the experimental diets for 8 weeks were significantly higher than those fed with the experimental diets supplemented with 2 weeks and the first 4 weeks Feed the experimental diets with serum SOD (P <0.05). The levels of glutathione peroxidase (GSH-Px) in hepatopancreas in Ala-Gln various feeding groups were significantly higher than those in control group (P <0.05). Serum GSH-Px was significantly higher in the experimental diets fed with the experimental diets at 4 weeks and the experimental diets fed for 8 weeks (P <0.05). Among the four Ala-Gln feeding groups, the LZM of head kidney was significantly higher than that of the control group (P <0.05) except for the 2w feeding group. The LZM of spleen in experimental feeding group and experimental feeding group at 4w after 4w were significantly higher than those in control group (P <0.05). NO in the head and kidney of the control group was significantly higher than that of the feeding groups (P <0.05). Under the experimental conditions, the addition of Ala-Gln to feed can increase the growth, antioxidation and immunity of Jian carp. There are also significant differences between feeding methods, from the perspective of growth, anti-oxidation and immunity, combined with economic and applicability considerations, we recommend the use of 2w interval feeding.